Home  Contacts  Order  Catalog  Support
 Search  Alphabetical Index  Numerical Index
 Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification
 Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents
C A T A L O G
 

HphI 

5'-G G T G A(N)8^-3'
3'-C C A C T(N)7^-5'

digested lambda DNA (dam-)
1.4% agarose
      
#ER1101
 Supplied with:
10X Buffer B
10X Buffer Tango™		 300 u
 
1 ml
1 ml
#ER1102
 Supplied with:
10X Buffer B
10X Buffer Tango™		 1500 u
 
1 ml
1 ml
Commercial Isoschizomers: AsuHPI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1101, #ER1102
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer B 37°C 0.1 65°C 100 0-20 0-20 0-20 20-50 0-20

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with HphI, more than 70% of the digested DNA fragments can be ligated and more than 90% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  may overlaps
 may overlap
 may overlap
 never overlaps
 may overlap		 - blocked.
- no effect.
- no effect.
- no effect.
- blocked.

Note
  • Assayed using lambda DNA (dam-) (#SD0021).
  • High glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer B 20-50 NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
168 9 18 12 7 7 6 6 6 17 16

See also General Properties of Restriction Enzymes:

 

 Home  Search  Contacts  Order  Catalog  Support

Updated kovo 07, 2008 14:46