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HindIII
Available in FastDigest® format

5'-A^A G C T T-3' 3'-T T C G A^A-5'		digested lambda DNA 0.7% agarose
#ER0501 Supplied with: 10X Buffer R 10X Buffer Tango™	5000 u   2x1 ml 1 ml
#ER0502 Supplied with: 10X Buffer R 10X Buffer Tango™	5x5000 u   2x1 ml 1 ml
#ER0503 Supplied with: 10X Buffer R 10X Buffer Tango™	HC, 25000 u   2x1 ml 1 ml
#ER0505 Supplied with: 10X Buffer R 10X Buffer Tango™	10000 u   4x1 ml 1 ml
Commercial Isoschizomers: -
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0501, #ER0502, #ER0503, #ER0505 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	37°C	0.1	65°C	0-20	20-50	0-20	100	50-100	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 250 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with HindIII, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  never overlaps  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - cleavage impaired.

Digestion of Agarose-embedded DNA
Mnimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	50-100	50-100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
7	0	1	1	1	1	1	1	1	1	1

Updated balandžio 22, 2008 11:26