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Hin1II (NlaIII)
 Available in FastDigest® format

5'- C A T G^-3'
3'-^G T A C -5'
digested lambda DNA
1.4% agarose
     
#ER1831
 Supplied with:
10X Buffer G
10X Buffer Tango™		 300 u
 
1 ml
1 ml
Commercial Isoschizomers:
(CviAII), FaeI, (FatI), Hsp92II, NlaIII
Enzymes in parentheses have different cleavage specificities.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1831
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer G 37°C 0.3 65°C 50-100 100 20-50 50-100 50-100 50-100

Storage Buffer
10 mM potassium phosphate (pH 7.4 at 25°C), 500 mM NaCl, 1 mM EDTA, 7 mM 2-mercaptoethanol, 0.15% Triton X-100, 0.5 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Hin1II, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 never overlaps
 may overlap		 - no effect.
- no effect.
- no effect.
- no effect.
- effect not determined.

Note

  • More stable when stored at -70°C.

  • At -20°C the half-life of Hin1II is 6 months.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer G 50-100 50-100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
181 22 15 26 11 11 9 8 8 16 23

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:25