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C A T A L O G
 

Hin1I (AcyI)
Available in FastDigest® format

5'-G Pu^C G Py C-3'
3'-C Py G C^Pu G-5'

digested lambda DNA
1.0% agarose
        
#ER0471
Supplied with:
10X Buffer G
10X Buffer Tango™		 300 u
 
1 ml
1 ml
#ER0472
Supplied with:
10X Buffer G
10X Buffer Tango™		 1500 u
 
1 ml
1 ml
Commercial Isoschizomers:
AcyI, BsaHIm, BssNI, BstACI, Hsp92I
"m" indicates different sensitivity to methylation.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0471, #ER0472
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer G 37°C 0.1 65°C 20-50 100 20-50 20-50 20-50 20-50

Storage Buffer
10 mM potassium phosphate (pH 7.4 at 25°C), 100 mM NaCl, 1 mM EDTA, 7 mM 2-mercaptoethanol, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Hin1I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 may overlap
 completely overlaps
 never overlaps
 may overlap		 - no effect.
- cleavage impaired.
- blocked.
- no effect.
- effect not determined.

Compatible Ends
AccI, AciI, AclI, Bsp119I, Bsu15I, ClaI, Hin6I, HinP1I, HpaII, MaeII, MspI, NarI, Psp1406I, SsiI, TaqI, XmiI.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer G 20-50 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
40 7 1 6 3 3 1 1 1 2 4

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:26