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HhaI
Available in FastDigest® format

5'-G C G^C-3' 3'-C^G C G-5'		digested lambda DNA 1.4% agarose
#ER1851 Supplied with: 10X Buffer Tango™	2000 u   1 ml
Commercial Isoschizomers: AspLEI, BstHHI, CfoI, (Hin6I), (HinP1I), (HspAI) Enzymes in parentheses have different cleavage specificities.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1851 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer Tango™	37°C	0.1	80°C	50-100	50-100	20-50	20-50	100	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with HhaI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Compatible Ends

Mva1269I -	GAATGCG^
	CTTAC^GC

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Tango™	100	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
215	18	26	31	17	17	20	24	24	16	26

Updated balandžio 22, 2008 11:26