Home  Contacts  Order  Catalog  Support
 Search  Alphabetical Index  Numerical Index
 Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification
 Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents
C A T A L O G
 

GsuI (BpmI) 

5'-C T G G A G(N)16^-3'
3'-G A C C T C(N)14^-5'

digested lambda DNA
1.0% agarose
      
#ER0461
Supplied with:
10X Buffer B
10X Buffer Tango™		 100 u
 
1 ml
1 ml
#ER0462
 Supplied with:
10X Buffer B
10X Buffer Tango™		 500 u
 
1 ml
1 ml
Commercial Isoschizomers: BpmIm
"m" indicates different sensitivity to methylation.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0461, #ER0462
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer B 30°C 1.0 65°C 100 50-100 20-50 20-50 100 50-100

Storage Buffer
10 mM potassium phosphate (pH 7.5 at 25°C), 1 mM EDTA, 7 mM 2-mercapthoethanol, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with GsuI, approximately 90% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 may overlap
 never overlaps
 never overlaps
 never overlaps		 - no effect.
- blocked.
- no effect.
- no effect.
- no effect.

Note
  • GsuI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.01 mM S-adenosylmethionine gives a 2-fold increase in GsuI activity.
  • Incubation at 37°C results in 70% activity.
  • GsuI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid an atypical DNA band pattern, use the 6X Loading Dye & SDS Solution (#R1151) for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer B 100 50-100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
25 3 2 4 1 1 1 1 2 1 4

See also General Properties of Restriction Enzymes:

 

 Home  Search  Contacts  Order  Catalog  Support

Updated kovo 06, 2008 15:35