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FastDigest® ScrFI (Bme1390I)*

* FastDigest® ScrFI (Bme1390I) is a proprietary formulation of Bme1390I,
an isoschizomer of ScrFI having the same recognition and
cleavage specificity.
5'-C C^N G G-3'
3'-G G N^C C-5'

1.4% agarose
Digested lambda DNA (dcm-) in 5 min with FastDigest® ScrFI (Bme1390I)
fast digest, fast digestion, time saver
      
#FD1424
Supplied with:
10X FastDigest® buffer		 100 reactions
 
1 ml

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #FD1424
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 834 KB)
Brochure (in pdf, 383 KB)
LabAid™ (in pdf, 97 KB)

 

Features

Unit Definition
One FastDigest® Unit (FDU) is the amount of the enzyme required to cleave 1 µg of lambda DNA dcm- in 5 min at 37°C in 1X FastDigest® buffer.

Concentration
1 FDU/µl.

Recommended
buffer

Reaction conditions
Temperature Time
FastDigest® buffer 37°C 5 min

Thermal Inactivation
Phenol/chloroform extraction and ethanol precipitation of DNA. Thermal inactivation is not applicable for FastDigest® ScrFI (Bme1309I).

Digestion of Plasmid DNA
1 µl of FastDigest® ScrFI (Bme1309I) digests up to 1 µg of plasmid DNA in 5 min, see Protocol for Fast Digestion and Analysis of Plasmid DNA with FastDigest® Enzymes and Protocol for Fast Vector Preparation with FastDigest® Enzymes.

Digestion of PCR Products
1 µl of FastDigest® ScrFI (Bme1309I) digests ~0.2 µg of PCR product in 5 min, see Protocol for Fast Digestion of PCR Products with FastDigest® Enzymes.

Digestion of Genomic DNA
1 µl of FastDigest® ScrFI (Bme1309I) digests 1 µg of genomic DNA in 5 min, or 5 µg of genomic DNA in 60 min, see Protocol for Fast Digestion of Genomic DNA with FastDigest® Enzymes.

Ligation and Recleavage
After overdigestion with 1 µl of FastDigest® ScrFI (Bme1309I) for 1 hour, more than 80% of the digested DNA fragments can be ligated in the reaction mixture containing 20-40 u of T4 DNA ligase/1 µg of fragments and 10% PEG. More than 95% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		 never overlaps
 may overlap
 may overlap
 never overlaps
 never overlaps		   - no effect.
- blocked.
- blocked.
- no effect.
- no effect.

Compatible Ends
CC^(C/G)GG - BcnI, NciI, SatI;
CC^(A/T)GG - Fnu4HI, MvaI, SatI.

Recommendations for Double and Triple Digestions with FastDigest® Enzymes

Perform 5 minute double or triple digestions with FastDigest® ScrFI (Bme1309I) and any other FastDigest® enzyme(s) in FastDigest® buffer, see Protocol for Double Digestion with FastDigest® Enzymes and Protocol for Triple Digestion with FastDigest® Enzymes.

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Updated vasario 05, 2008 15:39