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C A T A L O G
 

FastDigest® BglII

5'-A^G A T C T-3'
3'-T C T A G^A-5'

0.7% agarose
Digested lambda DNA in 5 min with FastDigest® BglII
fast digest, fast digestion, time saver
     
#FD0083
Supplied with:
10X FastDigest® buffer		 100 reactions
 
1 ml
#FD0084
Supplied with:
10X FastDigest® buffer		 200 reactions
 
1 ml

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #FD0083, #FD0084
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 834 KB)
Brochure (in pdf, 383 KB)
LabAid™ (in pdf, 97 KB)

 

Features

Unit Definition
One FastDigest® Unit (FDU) is the amount of the enzyme required to cleave 1 µg of lambda DNA in 5 min at 37°C in 1X FastDigest® buffer.

Concentration
1 FDU/µl.

Recommended
buffer

Reaction conditions
Temperature Time
FastDigest® buffer 37°C 5 min

Thermal Inactivation
Phenol/chloroform extraction and ethanol precipitation of DNA. Thermal inactivation is not applicable for FastDigest® BglII.

Digestion of Plasmid DNA
1 µl of FastDigest® BglII digests up to 0.6 µg of plasmid DNA in 5 min or 1 µg in 20 min, see Protocol for Fast Digestion and Analysis of Plasmid DNA with FastDigest® Enzymes and Protocol for Fast Vector Preparation with FastDigest® Enzymes.

Digestion of PCR Products
1 µl of FastDigest® BglII digests ~0.2 µg of PCR product in 30 min, see Protocol for Fast Digestion of PCR Products with FastDigest® Enzymes.

Digestion of Genomic DNA
1 µl of FastDigest® BglII digests 1 µg of genomic DNA in 20 min, or 5 µg of genomic DNA in 2 hours, see Protocol for Fast Digestion of Genomic DNA with FastDigest® Enzymes.

Ligation and Recleavage
After overdigestion with 1 µl of FastDigest® BglII for 1 hour, more than 95% of the DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  completely overlaps
 never overlaps
 never overlaps
 never overlaps
 may overlap		   - no effect.
- no effect.
- no effect.
- no effect.
- cleavage impaired.

Compatible Ends
BamHI, BclI, Bsp143I, MboI, PsuI.

Recommendations for Double and Triple Digestions with FastDigest® Enzymes

Perform 5 minute double or triple digestions with FastDigest® BglII and any other FastDigest® enzyme(s) in FastDigest® buffer, see Protocol for Double Digestion with FastDigest® Enzymes and Protocol for Triple Digestion with FastDigest® Enzymes.

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Updated kovo 17, 2008 14:06