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FastDigest® Alw21I

      A     A
5'-G T G C T^C-3'
3'-C^A C G A G-5'
     T     T

1.0% agarose
Digested lambda DNA in 5 min with FastDigest® Alw21I
fast digest, fast digestion, time saver
    
#FD0024
Supplied with:
10X FastDigest® buffer		 100 reactions
 
1 ml

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #FD0024
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 834 KB)
Brochure (in pdf, 383 KB)
LabAid™ (in pdf, 97 KB)

 

Features

Unit Definition
One FastDigest® Unit (FDU) is the amount of the enzyme required to cleave 1 µg of lambda DNA in 5 min at 37°C in 1X FastDigest® buffer.

Concentration
1 FDU/µl.

Recommended
buffer

Reaction conditions
Temperature Time
1X FastDigest® buffer 37°C 5 min

Thermal Inactivation
FastDigest® Alw21I is inactivated by incubation at 65°C for 5 min.

Digestion of Plasmid DNA
1 µl of FastDigest® Alw21I digests up to 1 µg of plasmid DNA in 5 min, see Protocol for Fast Digestion and Analysis of Plasmid DNA with FastDigest® Enzymes and Protocol for Fast Vector Preparation with FastDigest® Enzymes.

Digestion of PCR Products
1 µl of FastDigest® Alw21I digests ~0.2 µg of PCR product in 5 min, see Protocol for Fast Digestion of PCR Products with FastDigest® Enzymes.

Digestion of Genomic DNA
1 µl of FastDigest® Alw21I digests 1 µg of genomic DNA in 5 min, or 5 µg of genomic DNA in 30 min, see Protocol for Fast Digestion of Genomic DNA with FastDigest® Enzymes.

Ligation and Recleavage
After overdigestion with 1 µl of FastDigest® Alw21I for 1 hour, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 may overlap
 may overlap		 - no effect.
- no effect.
- no effect.
- no effect.
- effect not determined.

Compatible Ends
GTGCA^C - Bme1580I, BseSI, SduI, Mph1103I, NsiI, PstI, SbfI, SdaI.
GAGCT^C - Eco24I, SacI, SduI.
GAGCA^C - SduI.

Recommendations for Double and Triple Digestions with FastDigest® Enzymes

Perform 5 minute double or triple digestions with FastDigest® Alw21I and any other FastDigest® enzyme(s) in FastDigest® buffer, see Protocol for Double Digestion with FastDigest® Enzymes and Protocol for Triple Digestion with FastDigest® Enzymes.

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Updated vasario 05, 2008 15:41