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Eco130I (StyI)
Available in FastDigest® format

       A A
5'-C^C T T G G-3'
3'-G G A A C^C-5'
       T T

digested lambda DNA
1.0% agarose
       
#ER0411
Supplied with:
10X Buffer O
10X Buffer Tango™		 2500 u
 
1 ml
1 ml
Commercial Isoschizomers:
BssT1I, EcoT14I, ErhI, StyI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0411
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer O 37°C 0.2 65°C 0-20 20-50 100 50-100 50-100 100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Eco130I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- no effect.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
C^CTAGG - AvrII, BcuI, NciI, NheI, XbaI, XmaJI.
C^CATGG - AflIII, PscI, FatI, PagI, BtgI, NcoI.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer O 50-100 100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
10 0 0 1 0 0 0 0 0 0 2

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:22