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Eam1105I (AhdI)
Available in FastDigest® format

5'-G A C N N N^N N G T C-3' 3'-C T G N N^N N N C A G-5'		digested lambda DNA 0.7% agarose
#ER0241 Supplied with: 10X Buffer Eam1105I 10X Buffer Tango™	1000 u   1 ml 1 ml
#ER0242 Supplied with: 10X Buffer Eam1105I 10X Buffer Tango™	5000 u   2x1 ml 1 ml
Commercial Isoschizomers: AhdI, AspEI, BmeRI, DriI, EclHKI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0241, #ER0242 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				Eam1105I (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer Eam1105I	37°C	0.1	65°C	100	20-50	50-100	0-20	0-20	50-100	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recutting
After 10-fold overdigestion with Eam1105I, more than 80% of the digested DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  never overlaps  never overlaps	- no effect. - no effect. - no effect. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Eam1105I	50-100	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
9	1	0	1	1	1	1	1	1	1	1

Updated balandžio 22, 2008 11:22