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C A T A L O G
 

DpnI
Available in FastDigest® format

     CH3
5'-G A^T C-3'
3'-C T^A G-5'
       CH3

digested pBR322  DNA
1.4% agarose
      
#ER1701
Supplied with:
10X Buffer Tango™		 500 u
 
1 ml
#ER1702
Supplied with:
10X Buffer Tango™		 2500 u
 
1 ml
#ER1705
Supplied with:
10X Buffer Tango™		 1000 u
 
1 ml
Commercial Isoschizomers:
(BfuCI), (Bsp143I), (BssMI), (BstKTI), (BstMBI),
(DpnII), (Kzo9I), MalI, (MboI), (NdeII), (Sau3AI)
Enzymes in parentheses have different cleavage specificities.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1701, #ER1702, #ER1705
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 0.1 80°C 100 100 50-100 50-100 100 50-100

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 400 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with DpnI, more than 70% of the digested pBR322 DNA fragments can be ligated and more than 95% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  does not cut dam- DNA.
 never overlaps
 may overlap
 never overlaps
 may overlap
- no effect.
- no effect.
- no effect.
- effect not determined.

Note

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 50-100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
116 0 7 22 15 15 15  15 15 22 15

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:22