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CfrI (EaeI)

5'-Py^G G C C Pu-3'
3'-Pu C C G G^Py-5'

digested lambda DNA (dcm-)
1.0% agarose
        
#ER0161
 Supplied with:
10X Buffer Tango™		 200 u
 
1 ml
#ER0162
 Supplied with:
10X Buffer Tango™		 1000 u
 
1 ml
Commercial Isoschizomers: EaeI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0161, #ER0162
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 1.0 65°C 50-100* 50-100 0-20 0-20 100 0-20

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
10 mM Tris-HCl (pH 8.5 at 25°C), 500 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with CfrI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 may overlap
 may overlap
 never overlaps
 never overlaps		 - no effect.
- blocked.
- blocked.
- no effect.
- no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note
Assayed using lambda DNA (dcm-) (#SD0021).

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
39 2 3 6 3 3 3 4 4 4 8

See also General Properties of Restriction Enzymes:

 

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Updated kovo 05, 2008 16:47