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Cfr10I (BsrFI)
Available in FastDigest® format

5'-Pu^C C G G Py-3'
3'-Py G G C C^Pu-5'

digested lambda DNA
1.0% agarose
         
#ER0181
 Supplied with:
10X Buffer Cfr10I
10X Buffer Tango™		 200 u
 
1 ml
1 ml
#ER0182
 Supplied with:
10X Buffer Cfr10I
10X Buffer Tango™		 1000 u
 
1 ml
1 ml
Commercial Isoschizomers:
Bse118I, BsrFI, BssAIm
"m" indicates different sensitivity to methylation.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0181, #ER0182
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
Cfr10I
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer Cfr10I 37°C 0.1 No 100 0-20 20-50 20-50 50-100 20-50 50-100

Storage Buffer
10 mM potassium phosphate (pH 7.4 at 25°C), 100 mM KCl, 2 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 5-fold overdigestion with Cfr10I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 completely overlaps
 may overlap
 may overlap		 - no effect.
- no effect.
- blocked.
- effect not determined.
- effect not determined.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
ApeI, AvaI, BshTI, BsaWI, Cfr9I, Eco88I, Kpn2I, NgoMIV, SgrAI.

Note
Greater than 5-fold overdigestion with Cfr10I may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Cfr10I 20-50 50-100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
61 0 1 7 1 1 2 2 2 5 12

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:23