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BspLI (NlaIV)
Available in FastDigest® format

5'-G G N^N C C-3' 3'-C C N^N G G-5'		digested lambda DNA 1.4% agarose
#ER1151 Supplied with: 10X Buffer Tango™	200 u   1 ml
#ER1152 Supplied with: 10X Buffer Tango™	1000 u   1 ml
Commercial Isoschizomers: BmiI, NlaIVm, PspN4I "m" indicates different sensitivity to methylation.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1151, #ER1152 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer Tango™	37°C	0.3	65°C	50-100	50-100	0-20	20-50	100	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BspLI, approximately 90% of the digested DNA fragments can be ligated and more than 95% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  may overlap  may overlap  never overlaps  never overlaps	- no effect. - cleavage impaired. - cleavage impaired. - no effect. - no effect.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer Tango™	100	20-50

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
82	6	18	24	11	12	13	15	15	10	23

Updated balandžio 22, 2008 11:14