Home  Contacts  Order  Catalog  Support
 Search  Alphabetical Index  Numerical Index
 Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification
 Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents
C A T A L O G
 

Bsp143I (Sau3AI)

5'-^G A T C -3'
3'- C T A G^-5'

digested lambda DNA
1.4% agarose
     
#ER0781
Supplied with:
10X Buffer Bsp143I
10X Buffer Tango™		 300 u
 
1 ml
1 ml
#ER0782
Supplied with:
10X Buffer Bsp143I
10X Buffer Tango™		 1500 u
 
1 ml
1 ml
Commercial Isoschizomers:
BfuCI, BssMI, (BstKTIm), BstMBI, (DpnI), DpnIIm, Kzo9I,
(MalI), MboIm, NdeIIm, Sau3AIm
Enzymes in parentheses have different cleavage specificities.
"m" indicates different sensitivity to methylation.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0781, #ER0782
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
Bsp143I
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer Bsp143I 37°C 0.1 65°C 100 20-50 20-50 0-20 0-20 50-100 20-50

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Bsp143I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  completely overlaps
 never overlaps
 may overlap
 never overlaps
 may overlap		 - no effect.
- no effect.
- blocked.
- no effect.
- no effect.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Bsp143I 50-100 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
116 0 7 22 15 15 15 15 15 22 15

See also General Properties of Restriction Enzymes:

 

 Home  Search  Contacts  Order  Catalog  Support

Updated kovo 05, 2008 16:41