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Bsh1285I (BsiEI)

5'-C G Pu Py^C G-3' 3'-G C^Py Pu G C-5'		digested lambda DNA 1.0% agarose
#ER0891 Supplied with: 10X Buffer G 10X Buffer Tango™	600 u   1 ml 1 ml
Commercial Isoschizomers: BsiEI, BstMCI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0891 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer G	37°C	0.2	80°C	20-50	100	20-50	0-20	0-20	20-50

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 150 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Bsh1285I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	may overlap  never overlaps  completely overlaps  never overlaps  never overlaps	- no effect. - no effect. - blocked. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer G	NR	20-50

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
22	1	4	7	5	5	5	6	6	6	6

Updated vasario 29, 2008 12:08