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BseXI (BbvI) 

5'-G C A G C (N)8 ^-3'
3'-C G T C G (N)12^-5'

digested pBR322 DNA
1.4% agarose
     
#ER1451
Supplied with:
10X Buffer BseXI		 100 u
 
1 ml
#ER1452
Supplied with:
10X Buffer BseXI		 500 u
 
1 ml
Commercial Isoschizomers: BbvI, BstV1I

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1451, #ER1452
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
3 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
BseXI
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer BseXI 65°C 0.3 80°C 100 NR NR NR NR NR NR

NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with BseXI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 never overlaps
 may overlap		 - no effect.
- no effect.
- no effect.
- no effect.
- effect not determined.

Note
  • Incubation at 37°C results in 10% activity.
  • Assayed using pBR322 DNA (#SD0041).
  • Greater than 40-fold overdigestion with BseXI may result in the star activity.
  • BseXI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid an atypical DNA band pattern, use the 6X Loading Dye & SDS Solution (#R1151) for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis

Double Digestions using Tango™ Buffer

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer BseXI NR NR

NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
199 14 10 21 12 12 12 13 13 7 15

See also General Properties of Restriction Enzymes:

 

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Updated kovo 06, 2008 13:33