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BseSI (Bme1580I)
Available in FastDigest® format

G A 5'-G T G C C^C-3' 3'-C^A C G G G-5' C T		digested lambda DNA 0.7% agarose
#ER1441 Supplied with: 10X Buffer G 10X Buffer Tango™	500 u   1 ml 1 ml
#ER1442 Supplied with: 10X Buffer G 10X Buffer Tango™	2500 u   1 ml 1 ml
Commercial Isoschizomers: Bme1580I
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1441, #ER1442 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer G	55°C	0.1	80°C (10 u)	20-50	100	0-20	20-50	50-100	0-20

80°C(10 u) indicates that only small amounts of enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BseSI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  may overlap  may overlap  may overlap  never overlaps	- no effect. - no effect. - no effect. - cleavage impaired. - no effect.

Compatible Ends
GGGCC^C - ApaI, Eco24I, SduI.
GTGCA^C - Alw21I, Mph1103I, NsiI, PstI, SbfI, SdaI, SduI.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note

Incubation at 37°C results in 20% activity. Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer G	50-100	NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
10	1	2*	3	3	4	2	3	3	2	1

* According to our experimental data, BseSI has only one recognition site in M13mp18/19 DNA at position 2088.

Updated balandžio 22, 2008 11:15