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BseMII (BspCNI) 

5'-C T C A G (N)10^-3'
3'-G A G T C (N)8 ^-5'

digested lambda DNA
1% agarose
     
#ER1401
 Supplied with:
10X Buffer Tango™
50X SAM		 100 u
 
1 ml
0.1 ml
#ER1402
Supplied with:
10X Buffer Tango™
50X SAM		 500 u
 
1 ml
2x0.1 ml
Commercial Isoschizomers: (BspCNI)
Enzymes in parentheses have different cleavage specificities.

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1401, #ER1402
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
1 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ + 
0.01 mM S-adenosylmethionine		 55°C 0.5 80°C 50-100
(+SAM)		 50-100
(+SAM)		 50-100
(+SAM)		 50-100
(+SAM)		 100
(+SAM)		 50-100
(+SAM)

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with BseMII, more than 90% of the digested DNA fragments can be ligated but none of these can be recut due to the methylation of the recognition sequence by restriction enzyme.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- no effect.
- no effect.
- no effect.

Note
  • Incubation at 37°C results in 30% activity.
  • Requires SAM for activity. Sinefungin can replace SAM in the restriction reaction. In this case DNA is not methylated and more than 95% of the ligated BseMII fragments can be recut by this enzyme.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ + 
0.01 mM S-adenosylmethionine		 100
(+SAM)		 50-100
(+SAM)

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
79 10 23 7 5 5 4 4 4 13 8

See also General Properties of Restriction Enzymes:

 

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Updated kovo 05, 2008 16:45