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BseMI (BsrDI)

5'-G C A A T G N N^-3' 3'-C G T T A C^N N -5'		digested lambda DNA 0.7% agarose
#ER1261 Supplied with: 10X Buffer R 10X Buffer Tango™	100 u   1 ml 1 ml
#ER1262 Supplied with: 10X Buffer R 10X Buffer Tango™	500 u   1 ml 1 ml
Commercial Isoschizomers: Bse3DI, BsrDI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1261, #ER1262 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer R	55°C	0.3	80°C	0-20	20-50	0-20	100	50-100	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BseMI, more than 95% of the digested DNA fragments can be ligated and approximately 80% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - effect not determined.

Note
Incubation at 37°C results in 20% activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer R	50-100	50-100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
44	4	3	2	2	2	2	2	2	3	1

Updated kovo 06, 2008 13:32