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BseJI (BsaBI)

5'-G A T N N^N N A T C-3'
3'-C T A N N^N N T A G-5'
digested lambda DNA (dam-)
1.0% agarose
       
#ER1711
Supplied with:
10X Buffer O
10X Buffer Tango™		 2000 u
 
1 ml
1 ml
Commercial Isoschizomers:
BsaBI, Bse8I, MamI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1711
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer O 65°C 0.1 No NR 100* 100 NR NR 100*

* Star activity appears at a greater then 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, because of high star activity.

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with BseJI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  may overlap
 never overlaps
 may overlap
 never overlaps
 may overlap		 - blocked.
- no effect.
- no effect.
- no effect.
- effect not determined.

Note
  • Incubation at 37°C results in less than 10% activity.
  • Greater than 20-fold overdigestion with BseJI may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer O NR 100*

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
21 2 2 1 0 0 0 0 0 0 1

See also General Properties of Restriction Enzymes:

 

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Updated vasario 06, 2008 13:20