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Bme1390I (ScrFI)
Available in FastDigest® format

5'-C C^N G G-3' 3'-G G N^C C-5'		digested lambda DNA (dcm-) 1.4% agarose
#ER1421 Supplied with: 10X Buffer O 10X Buffer Tango™	500 u   1 ml 1 ml
#ER1422 Supplied with: 10X Buffer O 10X Buffer Tango™	2500 u   1 ml 1 ml
Commercial Isoschizomers: BmrFI, (BssKI), (BstSCI), MspR9I, ScrFI, (StyD4I) Enzymes in parentheses have different cleavage specificities.
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1421, #ER1422 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer O	37°C	0.2	80°C	20-50	50-100	100	50-100	50-100	50-100

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Bme1390I, more than 80% of the digested DNA fragments can be ligated in the reaction mixture containing 20-40 u of T4 DNA ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  may overlap  may overlap  never overlaps  never overlaps	- no effect. - blocked. - blocked. - no effect. - no effect.

Compatible Ends
CC^(C/G)GG - BcnI, NciI, SatI;
CC^(A/T)GG - Fnu4HI, MvaI, SatI.

Note
Assayed using lambda DNA (dcm-) (#SD0021). 

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer O	50-100	50-100

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
184	3	11	16	12	12	10	11	11	14	22

Updated balandžio 22, 2008 11:16