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BglII
Available in FastDigest® format

5'-A^G A T C T-3' 3'-T C T A G^A-5'		digested lambda DNA 0.7% agarose
#ER0081 Supplied with: 10X Buffer O 10X Buffer Tango™	500 u   1 ml 1 ml
#ER0082 Supplied with: 10X Buffer O 10X Buffer Tango™	2500 u   1 ml 1 ml
Commercial Isoschizomers: -
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0081, #ER0082 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer O	37°C	0.1	No	0-20	20-50	100	50-100	0-20	100

Storage Buffer
10 mM Tris-HCl (pH 8.2 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BglII, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	completely overlaps  never overlaps  never overlaps  never overlaps  may overlap	- no effect. - no effect. - no effect. - no effect. - cleavage impaired.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
BamHI, BclI, Bsp143I, MboI, PsuI.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer O	NR	100

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
6	0	1	0	0	0	0	0	0	0	0

 

See also General Properties for Restriction Enzymes: Reaction Conditions for Restriction Enzymes Five Buffer Plus System Stability During Prolonged Incubation Thermal Inactivation Double Digestion of DNA with Fermentas Enzymes Activity of Mesophilic and Thermophilic Enzymes at 37°C Site Preference by Restriction Enzymes Relaxation of Specificity (Star Activity) Sensitivity to Methylation Sensitivity to Dam and Dcm Methylation Sensitivity to CG Methylation Digestion of PCR Products Cleavage of PCR Products Directly After Amplification Reaction Cleavage Efficiency Close to the Termini of PCR Fragments Double Digestion of pUC19 Multiple Cloning Site Number of Recognition Sites in DNA Molecules  Troubleshooting

Updated balandžio 22, 2008 11:16