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BfuI (BciVI)

5'-G T A T C C (N)6^-3'
3'-C A T A G G (N)5^-5'

digested lambda DNA
0.7% agarose
      
#ER1501
Supplied with:
10X Buffer BfuI
10X Buffer Tango™		 100 u
 
1 ml
1 ml
#ER1502
Supplied with:
10X Buffer BfuI
10X Buffer Tango™		 500 u
 
1 ml
1 ml
Commercial Isoschizomers: BciVI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1501, #ER1502
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
BfuI
(unique)		 B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 1X 2X
Buffer BfuI 37°C 1.0 80°C 100 NR NR 0-20 0-20 NR 50-100*

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, because of high star activity.

Storage Buffer
 10 mM Tris-HCl (pH 7.4 at 25°C), 300 mM NaCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 5-fold overdigestion with BfuI, more than 80% of the digested DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA ligase/1 µg of fragments and 10% PEG. More than 90% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 may overlap
 may overlap
 never overlaps
 never overlaps		 - no effect.
- no effect.
- no effect.
- no effect.
- no effect.

Note
Greater than 5-fold overdigestion of with BfuI may result in star activity.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer BfuI NR 50-100*

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).
NR: buffer is not recommended, because of high star activity.

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
26 4 0 2 2 2 2 2 2 1 0

See also General Properties of Restriction Enzymes:

 

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Updated kovo 06, 2008 13:28