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C A T A L O G
 

BfmI (SfcI)
Available in FastDigest® format

5'-C^T Pu Py A G-3'
3'-G A Py Pu T^C-5'

digested lambda DNA
1.0% agarose
     
#ER1161
Supplied with:
10X Buffer Tango™		 200 u
 
1 ml
#ER1162
Supplied with:
10X Buffer Tango™		 1000 u
 
1 ml
Commercial Isoschizomers: BpcI, BstSFI, SfcI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1161, #ER1162
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 1.0 65°C 0-20 50-100 0-20 0-20 100 20-50

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BfmI, more than 95% of the digested DNA fragments can be ligated. More than 95% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 never overlaps
 never overlaps		 - no effect.
- no effect.
- no effect.
- no effect.
- no effect.

Compatible Ends
C^TGCAG - Alw44I.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity. 

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 20-50

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
38 6 7 4 4 4 6 6 6 1 1

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:17