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C A T A L O G
 

BcuI (SpeI)
Available in FastDigest® format

5'-A^C T A G T-3'
3'-T G A T C^A-5'

digested Ad2 DNA
0.7% agarose
       
#ER1251
Supplied with:
10X Buffer Tango™		 400 u
 
1 ml
#ER1252
 Supplied with:
10X Buffer Tango™		 2000 u
 
1 ml
Commercial Isoschizomers: AhlI, SpeI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER1251, #ER1252
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer Tango™ 37°C 0.5 No 50-100 50-100 0-20 20-50 100 0-20

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BcuI, more than 80% of the digested DNA fragments can be ligated and more than 90% of these can be recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 never overlaps
 may overlap
 may overlap		 - no effect.
- no effect.
- no effect.
- effect not determined.
- effect not determined.

Digestion of Agarose-embedded DNA
Minimum 10 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded Adenovirus-2 DNA in 16 hours.

Compatible Ends
AvrII, Eco130I, NheI, StyI, XbaI, XmaJI.

Note
Assayed using Adenovirus-2 DNA.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer Tango™ 100 NR

NR: buffer is not recommended, since enzyme activity is less than 20%.

Number of Recognition Sites in DNA Molecules

Ad2 Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
3 0 0 0 0 0 0 0 1 1 0 0

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:17