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BclI
Available in FastDigest® format

5'-T^G A T C A-3' 3'-A C T A G^T-5'		digested lambda DNA (dam-) 0.7% agarose
#ER0721 Supplied with: 10X Buffer G 10X Buffer Tango™	1000 u   1 ml 1 ml
#ER0722 Supplied with: 10X Buffer G 10X Buffer Tango™	5000 u   2x1 ml 1 ml
#ER0725 Supplied with: 10X Buffer G 10X Buffer Tango™	3000 u   2x1 ml 1 ml
Commercial Isoschizomers: FbaI, Ksp22I
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0721, #ER0722, #ER0725 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer G	55°C	0.1	80°C (10 u)	20-50	100	20-50	20-50	100*	100

 80°C(10 u) indicates that only small amounts of enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.
* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BclI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	completely overlaps  never overlaps  never overlap  never overlaps  may overlap	- blocked. - no effect. - no effect. - no effect. - blocked.

Compatible Ends
BamHI, BglII, Bsp143I, MboI, PsuI.

Note

Assayed using lambda DNA (dam-) (#SD0021). Incubation at 37°C results in 50% activity. Greater than 15-fold overdigestion with BclI may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer G	100*	100

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
8	0	0	0	0	0	0	0	0	0	1

Updated balandžio 22, 2008 11:17