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BamHI
Available in FastDigest® format

5'-G^G A T C C-3' 3'-C C T A G^G-5'		digested lambda DNA 0.7% agarose
#ER0051 Supplied with: 10X Buffer BamHI 10X Buffer Tango™	4000 u   2x1 ml 1 ml
#ER0052 Supplied with: 10X Buffer BamHI 10X Buffer Tango™	5x4000 u   4x1 ml 1 ml
#ER0053 Supplied with: 10X Buffer BamHI 10X Buffer Tango™	HC, 20000 u   4x1 ml 1 ml
#ER0055 Supplied with: 10X Buffer BamHI 10X Buffer Tango™	10000 u   4x1 ml 1 ml
Commercial Isoschizomers: -
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER0051, #ER0052, #ER0053, #ER0055 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl; 50 u/µl, HC

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				BamHI (unique)	B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer BamHI	37°C	0.5	80°C (10 u)	100	20-50*	100	20-50	50-100*	100*	50-100

80°C(10 u) indicates that only small amounts of enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.
* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 200 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton X-100, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with BamHI, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	completely overlaps  may overlap  may overlap  never overlaps  never overlaps	- no effect. - no effect. - no effect. - no effect. - no effect.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Compatible Ends
BclI, BglII, Bsp143I, MboI, PsuI.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity. 

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer BamHI	100*	50-100

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
5	1	1	1	1	1	1	1	1	1	1

Updated balandžio 22, 2008 11:17