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Alw21I (BsiHKAI)
Available in FastDigest® format

     A     A
5'-G T G C T^C-3'
3'-C^A C G A G-5'
     T     T

digested lambda DNA
1.0% agarose
     
#ER0021
Supplied with:
10X Buffer O
10X Buffer Tango™		 500 u
 
1 ml
1 ml
#ER0022
Supplied with:
10X Buffer O
10X Buffer Tango™		 2500 u
 
1 ml
1 ml
Commercial Isoschizomers: Bbv12I, BsiHKAI

Related Documents (in pdf, ~55 KB):

Certificate of Analysis: #ER0021, #ER0022
MSDS (English)
MSDS (English-USA)
MSDS (German)
Chart (in pdf, 721 KB)
Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended
buffer		 Incubation
temperature		 Units for 
overnight 
incubation,
u/µg DNA		 Thermal
inactivation,
in 20 min

Enzyme activity, %
B 
(blue)		 G 
(green)		 O 
(orange)		 R 
(red)		 Tango™ 
(yellow)
1X 1X 1X 1X 1X 2X
Buffer O 37°C 0.1 65°C 0-20 20-50 100 50-100 20-50 50-100

Storage Buffer
10 mM Tris-HCl (pH 7.4 at 25°C), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 50-fold overdigestion with Alw21I, more than 95% of the digested DNA fragments can be ligated and recut.

Methylation Effects
Dam:
Dcm:
CpG:
EcoKI:
EcoBI:		  never overlaps
 never overlaps
 may overlap
 may overlap
 may overlap		 - no effect.
- no effect.
- no effect.
- no effect.
- effect not determined.

Compatible Ends
GTGCA^C - Bme1580I, BseSI, SduI, Mph1103I, NsiI, PstI, SbfI, SdaI.
GAGCT^C - Eco24I, SacI, SduI.
GAGCA^C - SduI.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal
buffer

Enzyme activity, %
Tango™ 
(yellow)
1X 2X
Buffer O 20-50 50-100

Number of Recognition Sites in DNA Molecules

Lambda PhiX174 M13mp18/19 pBR322 pUC18/19 pUC57 pTZ19R/U pBluescriptIIKS(-/+) pBluescriptIISK(-/+) pACYC177 pACYC184
28 3 3 8 5 5 4 4 4 2 5

See also General Properties of Restriction Enzymes:

 

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Updated balandžio 22, 2008 11:18