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AjiI (BmgBI)

5'-C A C^G T C-3' 3'-G T G^C A G-5'		digested lambda DNA  0.7% agarose
#ER1941 Supplied with: 10X Buffer AjiI 10X Buffer Tango™	200 u   1 ml 1 ml
Commercial Isoschizomers: BmgBI, BtrI
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1941 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
5 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				AjiI (unique)	B (blue)	G (green)	O (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	1X	2X
Buffer AjiI	37°C	0.5	65°C	100	NR	NR	20-50*	NR	NR	20-50*

NR: buffer is not recommended, because of high star activity.
* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
AjiI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

Ligation and Recleavage
After 50-fold overdigestion with AjiI, approximately 80% of the digested DNA fragments can be ligated. No more than 50% of these can be recut due to the asymmetric recognition sequence of AjiI. The remaining uncleaved ligation products may be cut by AatII and Eco72I (PmlI).

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  completely overlaps  may overlap  may overlap	- no effect. - no effect. - cleavage blocked. - effect not determined. - effect not determined.

Digestion of Agarose-embedded DNA
Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note
Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity. 

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer AjiI	NR	20-50*

NR: buffer is not recommended, because of high star activity.
* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
17	0	0	0	0	0	0	0	0	0	0

Updated kovo 05, 2008 16:43