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AdeI (DraIII)
Available in FastDigest® format

5'-C A C N N N^G T G-3' 3'-G T G^N N N C A C-5'		digested lambda DNA 0.7% agarose
#ER1231 Supplied with: 10X Buffer G 10X Buffer Tango™	500 u   1 ml 1 ml
#ER1232 Supplied with: 10X Buffer G 10X Buffer Tango™	2500 u   1 ml 1 ml
Commercial Isoschizomers: DraIII
Related Documents (in pdf, ~55 KB): Certificate of Analysis: #ER1231, #ER1232 MSDS (English) MSDS (English-USA) MSDS (German) Chart (in pdf, 721 KB) Brochure (in pdf, 316 KB)

Concentration
10 u/µl

Conditions for 100% Activity

Recommended buffer	Incubation temperature	Units for  overnight  incubation, u/µg DNA	Thermal inactivation, in 20 min	Enzyme activity, %
				B  (blue)	G  (green)	O  (orange)	R  (red)	Tango™  (yellow)
				1X	1X	1X	1X	1X	2X
Buffer G	37°C	0.5	No	0-20	100	20-50	100	100*	20-50

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Storage Buffer
10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% glycerol.

Ligation and Recleavage
After 10-fold overdigestion with AdeI, more than 80% of the digested DNA fragments can be ligated and more than 90% of these sites can be recut.

Methylation Effects
Dam: Dcm: CpG: EcoKI: EcoBI:	never overlaps  never overlaps  may overlap  may overlap  may overlap	- no effect. - no effect. - cleavage impaired. - effect not determined. - effect not determined.

Digestion of Agarose-embedded DNA
Minimum 10 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

Note
Greater than 20-fold overdigestion of with AdeI may result in star activity.

Double Digestions using Tango™ Buffer, DoubleDigest™

Optimal buffer	Enzyme activity, %
	Tango™  (yellow)
	1X	2X
Buffer G	100*	20-50

* Star activity appears at a greater than 5-fold overdigestion (5 units x 1 hour).

Number of Recognition Sites in DNA Molecules

Lambda	PhiX174	M13mp18/19	pBR322	pUC18/19	pUC57	pTZ19R/U	pBluescriptIIKS(-/+)	pBluescriptIISK(-/+)	pACYC177	pACYC184
10	1	1	0	0	0	1	1	1	1	0

Updated balandžio 22, 2008 11:18