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PCR Master Mix (2X)

#K0171 Supplied with: Water, nuclease-free	4x1.25 ml for 200 reactions of 50 µl 4x1.25 ml
#K0172 Supplied with: Water, nuclease-free	20x1.25 ml for 1000 reactions of 50 µl 20x1.25 ml
Related Documents (in pdf, ~30-60 KB): Certificate of Analysis: #K0171, #K0172 MSDS (English) MSDS (English-USA) MSDS (German)

 

Features

• Convenient - Fermentas recombinant Taq DNA Polymerase in a ready-to-use mix.
• Fast -saves time due to reduced number of pipetting steps.
• Reproducible -lower contamination and pipetting error risk.
• Compatible with TA cloning - generates PCR products with 3'-dA overhangs.

Description
PCR Master Mix (2X) is a 2X concentrated solution of Taq DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Applications

• High throughput PCR.
• Routine PCR with high reproducibility.
• Generation of PCR products for TA cloning.
• RT-PCR.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.

Composition of the Master Mix

• 0.05 u/µl Taq DNA Polymerase (recombinant)
• reaction buffer
• 4 mM MgCl₂
• 0.4 mM of each dNTP (dATP, dCTP, dGTP, dTTP).

Stability of PCR Master Mix (2X). Amplification of 950 bp single copy gene from 0.1 µg human genomic DNA using PCR Master Mix (2X) in 50 µl reaction mixture.
M - GeneRuler™ 100 bp Plus DNA Ladder;
1 - PCR product generated with 1.25 u of Taq DNA Polymerase (recombinant);
2 - PCR product generated with PCR Master Mix (2X) (stored at -20°C);
3 - PCR product generated with PCR Master Mix (2X) (after 15 freeze-thaw cycles).

Related Products

FastRuler™ DNA Ladders, ready-to-use CloneJET™ PCR Cloning Kit InsTAclone™ PCR Cloning Kit Genomic DNA Purification Kit DNA Gel Extraction Kit GeneJET™ Plasmid Miniprep Kit First Strand cDNA Synthesis Kit RevertAid™ First Strand cDNA Synthesis Kits T4 DNA Polymerase Agarase

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights  (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel.  Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. For complete license disclaimer, see.

Updated balandžio 24, 2008 19:10