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Maxima™ SYBR Green qPCR Master Mix (2X)
High specificity of real-time PCR

#K0221 Supplied with: Water, nuclease-free	2x1.25 ml for 200 reactions of 25 µl 2x1.25 ml
#K0222 Supplied with: Water, nuclease-free	10x1.25 ml for 1000 reactions of 25 µl 10x1.25 ml
Related Documents (in pdf, ~30-60 KB): Certificate of Analysis: #K0221, #K0222 MSDS (English) MSDS (English-USA) MSDS (German) Flyer (in pdf, 243 KB)

 

Features

• Specificity - Maxima™ Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
• Sensitivity - detects low copy number targets.
• ROX - supplemented with ROX passive reference dye.
• Wide linear range - accurate quantification across 9 orders of magnitude.
• Universal - can be used on ABI and most real-time thermal cyclers.
• Reproducibility and convenience - ready-to-use 2X master mix.

Maxima™ SYBR Green qPCR Master Mix, flyer in pdf, 243 KB

Description
The Maxima™ SYBR Green qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step RT-PCR. The master mix includes Maxima™ Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It contains SYBR® Green dye and is supplemented with ROX passive reference dye. Only template and primers need to be added.
Maxima™ Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The SYBR® Green intercalating dye allows DNA detection and analysis without using sequence-specific probes. ROX serves as a passive reference dye.
The use of Maxima™ SYBR Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.

Applications

• Real-time PCR using SYBR® Green dye.
• Real-time RT-PCR using SYBR® Green dye.

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity and reproducibility using serial dilutions of control genomic DNA template.

Figure 1. Uniform and reproducible results.
Amplification of 10-fold dilutions of supercoiled plasmid DNA, starting from 10 ng down to 0.1 fg, using the Maxima™ SYBR Green qPCR Master Mix (2X) in duplicate reactions. Reactions were performed on the Eppendorf
Mastercycler® ep realplex instrument. The amplification plot and standard curve show the linearity across 9 orders of magnitude. NTC = non-template control.

Figure 2. Melting curve analysis confirms high qPCR specificity.
Amplification of 10-fold dilutions of supercoiled plasmid DNA, starting from 10 ng down to 0.1 fg, using the Maxima™ SYBR Green qPCR Master Mix (2X) in duplicate reactions. Reactions were performed on the Eppendorf Mastercycler® ep realplex instrument. NTC = non-template control.

Related Products

First Strand cDNA Synthesis Kit RevertAid™ First Strand cDNA Synthesis Kits M-MuLV Reverse Transcriptase RevertAid™ M-MuLV Reverse Transcriptase RevertAid™ H Minus M-MuLV Reverse Transcriptase Genomic DNA Purification Kit GeneJET™ Plasmid Miniprep Kit Uracil-DNA Glycosylase Water, nuclease-free

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights  (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel.  Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. For complete license disclaimer, see.

Updated kovo 04, 2008 10:15