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Maxima™ Probe qPCR Master Mix (2X)
High sensitivity real-time PCR

#K0231 Supplied with: Water, nuclease-free	2x1.25 ml for 200 reactions of 25 µl 2x1.25 ml
#K0232 Supplied with: Water, nuclease-free	10x1.25 ml for 1000 reactions of 25 µl 10x1.25 ml
Related Documents (in pdf, ~30-60 KB): Certificate of Analysis: #K0231, #K0232 MSDS (English) MSDS (English-USA) MSDS (German) Flyer (in pdf, 243 KB)

 

Features

• Sensitivity - detects low copy number targets.
• Specificity - Maxima™ Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
• ROX - supplemented with ROX passive reference dye.
• Wide linear range - accurate quantification across 9 orders of magnitude.
• Universal - can be used with sequence-specific probes on ABI and most real-time thermal cyclers.
• Reproducibility and convenience - ready-to-use 2X master mix.

Maxima™ Probe qPCR Master Mix, flyer in pdf, 243 KB

Description
The Maxima™ Probe qPCR Master Mix (2X) is a ready-to-use solution optimized for quantitative real-time PCR and two-step RT-PCR. The master mix includes Maxima™ Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It is supplemented with ROX passive reference dye. Only template and primers need to be added.
Maxima™ Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). ROX serves as a passive reference dye.
The use of Maxima™ Probe qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.

Applications

• Real-time PCR using sequence-specific probes.
• Real-time RT-PCR using sequence-specific probes.

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in qPCR for specificity, sensitivity and reproducibility using serial dilutions of control genomic DNA template.

Figure 1. Highly sensitive two step qRT-PCR.
Amplification of human PPP1CA gene was performed on serial 10-fold dilutions of Jurkat cell total RNA (from 1 ng to 1 pg). First strand cDNA was generated with the RevertAid™ First Strand cDNA Synthesis Kit. cDNA was amplified with the Maxima™ Probe qPCR Master Mix (2X) using the TaqMan® assay specific for PPP1CA. Reactions were performed on an ABI PRISM® 7000 instrument. 1 pg of total RNA was successfully detected. NTC = non-template control.

Figure 2. Precise and reproducible results.
Amplification of the human PGK1 gene was performed on serial 2-fold dilutions of human genomic DNA (from 0.5 µg to 1 ng) in 4 replicate reactions of 25 µl. Reactions were performed on an ABI PRISM® 7000 instrument. NTC = non-template control.

Related Products

First Strand cDNA Synthesis Kit RevertAid™ First Strand cDNA Synthesis Kits M-MuLV Reverse Transcriptase RevertAid™ M-MuLV Reverse Transcriptase RevertAid™ H Minus M-MuLV Reverse Transcriptase Genomic DNA Purification Kit GeneJET™ Plasmid Miniprep Kit Uracil-DNA Glycosylase Water, nuclease-free

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights  (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel.  Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. For complete license disclaimer, see.

Updated kovo 04, 2008 10:16