Primers for Sequencing
Product list:
The M13/pUC sequencing primers anneal to the region in the 5’-terminus of the lacZ gene. These primers are single-stranded oligonucleotides with 5’-hydroxyl and 3’-hydroxyl ends. All primers are supplied as 10 µM aqueous solutions.
M13/pUC sequencing primers Catalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Amount Concentration A260 units µg µl pmol µg/ml µM M13/pUC sequencing primer (-20), 17-mer
5'-d(GTAAAACGACGGCCAGT)-3'SO100 MSDS (English)
MSDS (English-USA)
MSDS (German)0.1 3.3 60 600 56.6 10 M13/pUC reverse sequencing primer (-26), 17-mer
5'-d(CAGGAAACAGCTATGAC)-3'SO101 MSDS (English)
MSDS (English-USA)
MSDS (German)60 600 56.6 M13/pUC sequencing primer (-40), 17-mer
5'-d(GTTTTCCCAGTCACGAC)-3'SO113 MSDS (English)
MSDS (English-USA)
MSDS (German)60 600 56.6 M13/pUC sequencing primer (-46), 22-mer
5'-d(GCCAGGGTTTTCCCAGTCACGA)-3'SO114 MSDS (English)
MSDS (English-USA)
MSDS (German)45 450 73.2 M13/pUC reverse sequencing primer (-46), 24-mer
5'-d(GAGCGGATAACAATTTCACACAGG)-3'SO115 MSDS (English)
MSDS (English-USA)
MSDS (German)42 420 79.9 Applications
- Sequencing of DNA fragments inserted into the MCS within the lacZ gene of various cloning vectors, such as pUC19, pTZ19R, pTZ57R, M13mp18, pBluescript II.
- Colony screening by PCR.
Quality Control
Functionally tested by dideoxy sequencing of pUC19 DNA.Store at -20°C
pJET1.2 Sequencing Primers new
The pJET1.2 sequencing primers flank the Eco32I site in the eco47IR gene of positive selection cloning vector pJET1.2. These primers are single-stranded oligonucleotides with 5’-hydroxyl and 3’-hydroxyl ends. All primers are supplied as 10 µM aqueous solutions.
pJET1.2 sequencing primers Catalog
#Product
resources
(in pdf)Amount,
A260 unitsConcentration,
µMpJET1.2 forward sequencing primer, 23-mer
5'-CGACTCACTATAGGGAGAGCGGC-3'SO501 0.2 10 pJET1.2 reverse sequencing primer, 24-mer
5'-AAGAACATCGATTTTCCATGGCAG-3'SO511 Applications
- Sequencing of DNA fragments inserted into Eco32I site within the eco47IR gene of pJET1.2.
- Colony screening by PCR.
Quality Control
Functionally tested by dideoxy sequencing of pJET1.2 DNA.Store at -20°C
Transcription Promoter Primers
The primers are complementary to the SP6, T3 or T7 RNA polymerase promoter regions respectively. These primers are single-stranded oligonucleotides with 5’- and 3’-hydroxyl ends. All primers are supplied as 10 µM aqueous solutions.
pJET1 sequencing primers Catalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Amount Concentration A260 units µg µl pmol µg/ml µM SP6 promoter sequencing primer, 18-mer
5'-d(ATTTAGGTGACACTATAG)-3'SO116 MSDS (English)
MSDS (English-USA)
MSDS (German)0.1 3.3 56 560 59.9 10 SP6 promoter sequencing primer, 24-mer
5'-d(CATACGATTTAGGTGACACTATAG)-3'SO117 MSDS (English)
MSDS (English-USA)
MSDS (German)42 420 79.9 T7 promoter sequencing primer, 20-mer
5'-d(TAATACGACTCACTATAGGG)-3'SO118 MSDS (English)
MSDS (English-USA)
MSDS (German)50 500 66.6 T3 promoter sequencing primer, 17-mer
5'-d(ATTAACCCTCACTAAAG)-3'SO119 MSDS (English)
MSDS (English-USA)
MSDS (German)60 600 56.6 T3 promoter sequencing primer, 24-mer
5'-d(GCGCGAAATTAACCCTCACTAAAG)-3'SO120 MSDS (English)
MSDS (English-USA)
MSDS (German)42 420 79.9 Applications
Sequencing of DNA fragments located downstream from the corresponding RNA polymerase promoter sequences in popular cloning vectors, such as pTZ19R, pTZ57R, pBluescript II.Quality Control
Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence.Note
Primers cannot be used for certain plasmids that contain truncated promoters lacking the primer binding sites.Store at -20°C
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Updated balandžio 24, 2008 19:09
