Home  Contacts  Order  Catalog  Support  Search  Alphabetical Index  Numerical Index  Restriction Enzymes  Modifying Enzymes  PCR, qPCR, RT-PCR & dNTPs  Molecular Cloning  Nucleic Acid Purification  Molecular Labeling & Detection  In vitro Transcription  Electrophoresis Products  Nucleotides  Transfection Reagents  Reagents C A T A L O G

Exo-Resistant Random Primer*
5’-NpNpNpNpNp^SNp^SN-3’

* Use of this product in certain applications may be covered by patents and may require a license.

#SO181	100 µl
Related Documents (in pdf, ~40-50 KB): Certificate of Analysis: #SO181 MSDS (English) MSDS (English-USA) MSDS (German)

 

Feature
3’-terminal PTO modifications protect from 3'=>5' exonuclease degradation and ensure efficient priming of DNA synthesis

Description
The Exo-Resistant Random Primer is a mixture of single-stranded random oligonucleotides used for highly efficient random priming of various DNA synthesis reactions. This primer has two 3’-terminal phosphorothioate (PTO) modifications that are resistant to the 3'=>5' exonuclease activity of proofreading DNA polymerases (1), like Klenow Fragment and phi29 DNA Polymerase. It also has 5’- and 3’-hydroxyl ends. The Exo-Resistant Random Primer is supplied as a ready-to-use, 20X concentrated aqueous solution.

Applications

• Strand displacement amplification of genomic DNA (2), plasmids and phage DNA (3).
• DNA labeling by random priming (4-6).

Concentration
500 µM (1.1 µg/µl)

Quality Control
Functionally tested for the efficient priming of DNA synthesis using phi29 DNA Polymerase.

References

1. Skerra, A., Phosphorothioate primers improve the amplification of DNA sequences by DNA polymerases with proofreading activity, Nucleic Acids Res., 20, 3551-3554, 1992.
2. Dean, F.B., et al., Comprehensive human genome amplification using multiple displacement amplification, Proc. Natl. Acad. Sci., 99, 5261-5266, 2002.
3. Dean, F.B., et al., Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification, Genome Res., 11, 1095-1099, 2001.
4. Feinberg, A.P. and Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem., 132, 6-13, 1983.
5. Feinberg, A.P. and Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Addendum, Anal. Biochem., 137, 266-267, 1984.
6. Mackey, J., et al., Use of random primer extension for concurrent amplification and nonradioactive labeling of nucleic acids, Anal. Biochem., 212, 428-435, 1993.