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ddNTP Sets, molecular biology grade

 
#R0072 4x1 µmol
(4x0.2 ml of 5 mM solution)
#R0073 4x1 µmol
(4x0.05 ml of 20 mM solution)

Related Documents (in pdf, ~30-50 KB):

Certificate of Analysis & MSDS:
#R0072,
MSDS (English)
MSDS (English-USA)
MSDS (German)

#R0073,
MSDS (English)
MSDS (English-USA)
MSDS (German)

Brochure (183 KB)

Description
Convenient Sets of either 5 mM or 20 mM solutions of each ddATP, ddCTP, ddGTP and ddTTP.

Applications
For dideoxy sequencing of DNA.

General Characteristics

  • ddATP
    C10H13N5O11P3Na3,
    MW = 541.2 (475.0)
    Absorbance maximum at 261nm,
    extinction coefficient = 15.2x103 M-1 x cm-1 (pH 7.0).
  • ddCTP
    C9H13N3O12P3Na3,
    MW = 517.1 (451.0)
    Absorbance maximum at 271nm,
    extinction coefficient = 9.3x103 M-1 x cm-1 (pH 7.0).
  • ddGTP
    C10H13N5O12P3Na3,
    MW = 557.1 (491.0)
    Absorbance maximum at 253nm, 
    extinction coefficient = 13.6x103 M-1 x cm-1 (pH 7.0).
  • ddTTP
    C10H14N2O13P3Na3,
    MW = 532.0 (466.0)
    Absorbance maximum at 267nm, 
    extinction coefficient = 15.2x103 M-1 x cm-1 (pH 7.0).

Quality Control
  • Functionally tested in cycle sequencing with Reader™ Taq DNA Polymerase.
  • Purity of >95% by HPLC of each component.
  • Endo-, exodeoxyribonuclease and phosphatase free.

Storage
Store at -20°C.

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Updated balandžio 24, 2008 19:03