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Biotin-11-dUTP, molecular biology grade

 
#R0081 50 nmol
(50 µl of 1 mM solution)
Formula

C28H41N6O17P3SNa3

Molecular Weight

927.6 (acid form: 861.6)

Related Documents (in pdf, ~30-50 KB):

Certificate of Analysis: #R0081
MSDS (English)
MSDS (English-USA)
MSDS (German)
Brochure (183 KB)

Description
Biotin-11-dUTP (biotin-e-aminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5'-triphosphate]) is supplied as a 1 mM aqueous solution at pH 7.0.

General Characteristics
Absorbance maximum at 240nm, extinction coefficient - 10.7x103 M-1 x cm-1 (pH 7.0);
Absorbance maximum at 289nm,  extinction coefficient - 7.1x103 M-1 x cm-1 (pH 7.0)

Applications

  • Enzymatic non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions.

  • Can be incorporated into DNA using Reverse Transcriptases, Taq DNA Polymerase, Klenow Fragment.

Quality Control
  • Functionally tested:
    • in PCR with Taq DNA Polymerase, 
    • in DNA labeling with Klenow fragment.
  • Purity of >96% by HPLC.
  • Endo-, exodeoxyribonuclease, ribonuclease and phosphatase free.
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Updated balandžio 24, 2008 19:03