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Aminoallyl-UTP, 50 mM Solution, molecular biology grade

#R1091	2.5 µmol (0.05 ml of 50 mM solution)
Formula	C12H17N3O15P3Na3
Molecular Weight	605.2 (acid form: 539.2)
Related Documents (in pdf, ~30-50 KB): Certificate of Analysis: #R1091 MSDS (English) MSDS (English-USA) MSDS (German) Brochure (183 KB)

Description
Aminoallyl-UTP (5-[3-aminoallyl]-uridine-5'-triphosphate) is supplied as a 50 mM aqueous solution at pH 7.0.

General Characteristics
Absorbance maximum at 240nm, extinction coefficient - 11.9x10³ M^-1 x cm^-1 (pH 7.0);
Absorbance maximum at 290nm, extinction coefficient - 7.8x10³ M^-1 x cm^-1 (pH 7.0).

Features

Can be incorporated into RNA by T3, T7 or SP6 RNA Polymerases. The resulting amine-containing RNA can be subsequently labeled with amine-reactive fluorescent dyes or haptens, resulting in uniform high density labeling of RNA.

Application

Enzymatic indirect non-radioactive labeling of RNA by in vitro transcription.

Quality Control

Functionally tested in transcription reaction with T7 RNA Polymerase. Purity of >96% by HPLC. Endo-, exodeoxyribonuclease, ribonuclease and phosphatase free.

Updated vasario 06, 2008 09:03