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Aminoallyl-dUTP, molecular biology grade

#R1101	2.5 µmol (0.05 ml of 50 mM solution)
#R0091	1 µmol (0.1 ml of 10 mM solution)
Formula	C12H17N3O14P3Na3
Molecular Weight	589.2 (acid form: 523.2)
Related Documents (in pdf, ~30-50 KB): Certificate of Analysis & MSDS: #R1101; MSDS (English) MSDS (English-USA) MSDS (German) #R0091; MSDS (English) MSDS (English-USA) MSDS (German) Brochure (183 KB)

Description
Aminoallyl-dUTP (5-[3-aminoallyl]-2'-deoxyuridine-5'-triphosphate) is supplied as a 50 mM and 10 mM aqueous solutions at pH 7.0.

General Characteristics
Absorbance maximum at 240nm, extinction coefficient - 11.9x10³ M^-1 x cm^-1 (pH 7.0);
Absorbance maximum at 290nm, extinction coefficient - 7.8x10³ M^-1 x cm^-1 (pH 7.0)

Features

Can be incorporated into DNA by Reverse Transcriptases, Taq DNA Polymerase, Klenow Fragment. The resulting aminoallyl-containing DNA is subsequently labeled with amine-reactive fluorescent dyes or haptens, resulting in uniform high density labeling of DNA.

Application

Enzymatic indirect non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions.

Quality Control

Functionally tested in cDNA synthesis with a ReverAid™ H Minus Reverse Transcriptase. Purity of >96% by HPLC. Endo-, exodeoxyribonuclease, ribonuclease and phosphatase free.

Updated balandžio 24, 2008 19:02