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pBR322 DNA

#SD0041	100 µg
Related Documents (in pdf, ~40-50 KB): Certificate of Analysis: #SD0041 MSDS (English) MSDS (English-USA) MSDS (German) Other Phages and Plasmids

Description
pBR322 DNA is a commonly used plasmid cloning vector in E.coli.
pBR322 DNA is isolated from E.coli (dam+, dcm+) by ion exchange chromatography.
The molecule is a double-stranded circle, 4361 base pairs in length.
For detailed information see the restriction map and sites.

Molecular Weight
2.83 x 10⁶ daltons.

Concentration
0.5 µg/µl.

Storage Buffer
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.

Quality Control

• More than 90% is in the supercoiled form.

• Gel analysis for purity and homogeneity of AluI, BsuRI and HindIII fragmentation patterns.
   

Updated vasario 06, 2008 08:58