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Lambda DNA (dam^-, dcm^-)

#SD0021	500 µg
Related Documents (in pdf, ~40-50 KB): Certificate of Analysis: #SD0021 MSDS (English) MSDS (English-USA) MSDS (German) Other Phages and Plasmids

Description
Lambda DNA (dam-, dcm-) is commonly used as a substrate for restriction enzymes sensitive to Dam or Dcm methylation. The phage is isolated from a heat-inducible lysogenic E.coli GM2163 (cI857 Sam7) strain. The DNA is isolated from purified phage by standard purification procedure.
The molecule is linear a double-stranded DNA, 48502 base pairs in length.
For detailed information see.

Molecular Weight
31.5 x 10⁶ daltons.

Feature
Used as a substrate for restriction enzymes sensitive to Dam or Dcm methylation.

Concentration
0.3 µg/µl.

Storage Buffer
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.

Quality Control
Gel analysis for purity and homogeneity of BclI, Eco31I, TaqI and XbaI fragmentation patterns.
 

Updated vasario 06, 2008 08:57