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DreamTaq™ DNA Polymerase

#EP0701 Supplied with: 10X DreamTaq™ Buffer 25 mM MgCl2	200 u (5 u/µl) (for 160 reactions of 50 µl) 1.25 ml 0.6 ml
#EP0702 Supplied with: 10X DreamTaq™ Buffer 25 mM MgCl2	500 u (5 u/µl) (for 400 reactions of 50 µl) 2x1.25 ml 1.25 ml
#EP0703 Supplied with: 10X DreamTaq™ Buffer 25 mM MgCl2	5x500 u (5 u/µl) (for 2000 reactions of 50 µl) 10x1.25 ml 5x1.25 ml
#EP0704 Supplied with: 10X DreamTaq™ Buffer 25 mM MgCl2	20x500 u (5 u/µl) (for 8000 reactions of 50 µl) 40x1.25 ml 20x1.25 ml
Larger packages and custom formulations available on request
Related Documents (in pdf, ~40-60 KB): Certificate of Analysis: #EP0701, #EP0702, #EP0703, #EP0704 MSDS (English) MSDS (English-USA) MSDS (German)
Troubleshooting (in pdf, 53 KB)

 

Features

• Robust amplification with minimal optimization.
• High yields of PCR products.
• Higher sensitivity compared to conventional Taq DNA polymerase.
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
• Generates 3'-dA overhangs.

Description
DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ DNA Polymerase uses the same reaction set-up and cycling conditions as conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required. It is supplied with optimized DreamTaq™ buffer, which includes 20 mM MgCl₂.
DreamTaq™ DNA Polymerase generates PCR products with 3'-dA overhangs. It does not incorporate dUTP.

Applications

• Routine PCR amplification of DNA fragments up to 6 kb.
• RT-PCR.
• Generation of PCR products for TA cloning.

Concentration
5 u/µl

Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested in PCR.

Definition of Activity Unit
One unit of the enzyme catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction (adsorbed on DE-81) in 30 min at 70°C.
Enzyme activity is assayed in the following mixture: 67 mM Tris-HCl (pH 8.8 at 25°C), 6.7 mM MgCl₂, 1 mM 2-mercaptoethanol, 50 mM NaCl, 0.1 mg/ml BSA, 0.75 mM activated calf thymus DNA, 0.2 mM of each dNTP, 0.4 MBq/ml [³H]-dTTP.

Storage Buffer
The enzyme is supplied in: 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycerol.

Inhibition and Inactivation

• Inhibitors: ionic detergents (deoxycholate, sarkosyl and SDS) at concentrations higher than 0.06, 0.02 and 0.01%, respectively (1).
• Inactivated by phenol/chloroform extraction.

Figure 1. Comparison of PCR sensitivity and yield with DreamTaq™ DNA Polymerase and other commercial Taq DNA polymerases.
A 545 bp fragment of human alfa-L-Fucosidase gene DNA was amplified according to manufacturers’ recommendations using decreasing amounts of human genomic DNA.
M - FastRuler™ Low Range DNA Ladder, ready-to-use
D - DreamTaq™ DNA Polymerase
T - Taq DNA Polymerase
1-3 - Taq DNA polymerases from different vendors

Related Products

dNTPs dNTP Mixes dNTP Set CloneJET™ PCR Cloning Kit InsTAclone™ PCR Cloning Kit First Strand cDNA Synthesis Kit RevertAid™ First Strand cDNA Synthesis Kits M-MuLV Reverse Transcriptase RevertAid™ M-MuLV Reverse Transcriptase RevertAid™ H Minus M-MuLV Reverse Transcriptase FastRuler™ DNA Ladders, ready-to-use GeneRuler™ DNA Ladders T4 DNA Polymerase Genomic DNA Purification Kit DNA Gel Extraction Kit Agarase GeneJET™ Plasmid Miniprep Kit Primers Water, nuclease-free

Reference

1. Weyant, R.S., et al., Effect of ionic and nonionic detergents on the Taq polymerase, BioTechniques, 9, 308-309, 1990.

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research. No other patent rights  (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel.  Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. For complete license disclaimer, see.

Updated kovo 18, 2008 09:08