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T7 Transcription Kit

#K0411	for 10 reactions (of 50 µl)
#K0412	for 30 reactions (of 50 µl)
Related Documents (in pdf, ~50-90 KB): Complete Protocol: #K0411, #K0412 MSDS (English) MSDS (English-USA) MSDS (German)

 

Features

• Effective - high specific activity of RNA probes.
• In vitro transcription from DNA templates with a T7 promoter.
• Convenient - includes the pTZ19R cloning vector, which contains a T7 promoter for in vitro transcription of inserts.
• Complete - RiboLock™ RNase Inhibitor is supplied with the kit.

Description
The T7 Transcription Kit uses recombinant T7 RNA polymerase for in vitro synthesis of radioactive RNA probes with high specific activity and well-defined RNA transcripts in microgram quantities. The kit includes all components necessary for in vitro transcription of DNA inserts cloned under the T7 promoter. The cloning vector pTZ19R included in the kit contains the T7 promoter region, as well as a convenient multiple cloning site. The yield of the transcript and the specific activity of the probe can be maximized using the kit components. The high purity of all components guarantees the stability of synthesized RNA during transcription reactions.

Applications

• Generation of radioactive RNA probes with high specific activity.
• Generation of microgram quantities of RNA transcripts of defined length.

Quality Control
The kit is tested in the production of high quality RNA transcripts using a control template during both 2 hour and 18 hour in vitro transcriptions.

Components of the Kit

• T7 RNA Polymerase
• 5X Transcription Buffer
• RiboLock™ RNase Inhibitor
• ATP Solution
• CTP Solution
• GTP Solution
• UTP Solution
• Vector pTZ19R DNA
• Control Template
• DEPC-treated Water
• Detailed Protocol (in pdf, ~91 KB):
  • #K0411
  • #K0412

In vitro transcription of cloned insert. pTZ19R vector.

Related Products

RiboRuler™ RNA Ladders 2X RNA Loading Dye Aminoallyl-UTP NTP Set RNA Polymerases RiboLock™ RNase Inhibitor pTZ19R InsTAclone™ PCR Cloning Kit Pyrophosphatase, Inorganic DNaseI, RNase-free T4 RNA Ligase T4 Polynucleotide Kinase FastAP™ Thermosensitive Alkaline Phosphatase Shrimp Alkaline Phosphatase (SAP) Rapid DNA Ligation Kit Sequencing Primers DEPC-treated Water

Updated birželio 30, 2008 13:02