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PageSilver™ Silver Staining Kit
Highly sensitive and fast staining of proteins and nucleic acids

#K0681	for 25 mini-gels
Related Documents (in pdf, 50 KB): Detailed Protocol: #K0681 MSDS (English) MSDS (English-USA) MSDS (German) QuickProtocol™ (in pdf, 58 KB)

 

Features

• High sensitivity - 0.05ng of protein per band can be detected.
• Clear background.
• Fast staining - procedure takes only 60 minutes.
• Compatible with in situ zymography.

Figure 1. High sensitivity of staining with PageSilver™ Silver Staining Kit. Different amounts of BSA were run on 4-12% Tris-glycine SDS-PAGE and stained with the PageSilver™ Silver Staining Kit according to protocol for maximum sensitivity.

Figure 2. Highly sensitive protein staining in clear background with PageSilver™ Silver Staining Kit. Different amounts of cell lysates were separated on 4-12% Tris-glycine SDS-PAGE and stained with the PageSilver™ Silver Staining Kit. M - PageRuler™ Plus Prestained Protein Ladder 1, 4 - 500 ng of total protein 2, 5 - 200 ng of total protein 3, 6 - 100 ng of total protein

Description
The PageSilver™ Silver Staining Kit is a complete system for rapid and sensitive staining of proteins, DNA and RNA in polyacrylamide gels. The staining procedure is fast and simple and can be completed in approximately 60 minutes with very little hands-on time. The composition of all components is optimized for highly sensitive protein staining (Fig.1). Approximately, 0.05-0.6 ng of protein per band can be visualized in an essentially clear background (Fig.2). This is >100 times more sensitive than traditional Coomassie Brilliant Blue R250 staining and allows for superior detection of low abundance proteins.
The PageSilver™ Silver Staining Kit is based on the use of silver nitrate to bind proteins at a weakly acidic pH and subsequent reduction of silver ions to metallic silver by formaldehyde at an alkaline pH (1, 2).

Applications

• Highly sensitive staining of proteins, DNA and RNA in polyacrylamide gels.
• Staining both non-denaturing polyacrylamide gels and denaturing polyacrylamide gels containing SDS or urea.
• Visualization of nanogram quantities of DNA and RNA.

Quality Control
The kit is tested using staining protocol for maximum speed, different dilutions of BSA (0.1-2.0 ng) are electrophoresed on a 1.0 mm, 12% Tris-glycine SDS gel. Silver staining detects 0.1ng BSA in clear background.

Note
Subsequent analysis of stained protein bands by mass spectrometry is not recommended.

Components of the Kit

• Sensitizer concentrate
• Staining reagent
• Developing reagent
• Formaldehyde
• Stop reagent
• Detailed Protocol (in pdf, 50 KB):
  • #K0681

Related Products

PageRuler™ Unstained Protein Ladder PageRuler™ Prestained Protein Ladder PageRuler™ Plus Prestained Protein Ladder Unstained Protein Molecular Weight Marker Prestained Protein Molecular Weight Marker PageBlue™ Protein Staining Solution 10X Tris-glycine-SDS Electrophoresis Buffer 10X Tris-glycine Electrophoresis Buffer 10X Tris-tricine-SDS Electrophoresis Buffer Protein Loading Buffer Pack

References

1. Rabilloud, T. et al., Silver-staining of proteins in polyacrylamide gels: a general overview, Cell. Mol. Biol., 40, 57-75, 1994. Cell. Mol. Biol. 40, 57-75, 1994.
2. Blum, H., Beier, H. And Gross, H.J., Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels, Electrophoresis, 8, 93-99, 1987.

Updated vasario 28, 2008 16:25