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Genomic DNA Purification Kit

#K0512	for 100 preps The prep uses: 200 µl of whole blood 25-30mg of mammalian tissue 50-100mg of plant tissue 0.4-0.6x106 of cultured cells or  10-20mg of bacteria
Related Documents (in pdf, ~30-50 KB): Detailed Protocol: #K0512 MSDS (English) MSDS (English-USA) MSDS (German) QuickProtocol™

 

Features

• Efficient - 2-10 µg of genomic DNA from 0.2 ml of blood.
• Fast - procedure takes only 20 min.
• Universal - purifies genomic DNA from various sources.
• Safe - no phenol extraction step.
• Convenient - purified DNA can be directly used in all molecular biology applications.
• Flexible - easy to scale up and down.

DNA purification from whole blood with Genomic DNA Purification Kit

Description
The Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from various sources: whole blood, serum, cell cultures, bacteria, plant and mammalian tissues, as well as from epithelial samples. The kit is based on selective DNA precipitation from crude lysates using detergents. The entire procedure takes only 20-25 minutes. The typical yield of genomic DNA is 2-10 µg from 0.2 ml of blood. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.

Applications
Purification of high-quality genomic DNA from whole blood, serum, cell culture, plant and mammalian tissues, epithelium samples and bacteria.

Quality Control
The kit is tested in purification of genomic DNA from 0.2 ml of human blood. The typical yield of DNA is 2-10 µg. The quality of purified DNA is evaluated in restriction digestion and in PCR amplification of a single-copy gene.

Components of the Kit

• Lysis Solution
• Precipitation Solution
• NaCl Solution
• Detailed Protocol (in pdf, ~29 KB):
  • #K0512

Agarose gel (0.7%) electrophoresis of genomic DNA from various sources purified with the Genomic DNA Purification kit.
DNA samples were analyzed undigested (odd lane numbers) and after digestion with EcoRI (even lane numbers).
M - lambda DNA/HindIII
1, 2 - human blood
3, 4 - human lymphocites
5, 6 - human placenta
7, 8 - mouse liver
9, 10 - Hedera helix
11, 12 - Haemophilus influenzae
13, 14 - E.coli ER2267
15, 16 - Bacillus subtilis

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Updated vasario 05, 2008 14:12