Biotin Chromogenic Detection Kit
#K0661 for 10 reactions #K0662 for 30 reactions Related Documents (in pdf, ~40 KB):
Detailed Protocol: #K0661, #K0662
MSDS (English)
MSDS (English-USA)
MSDS (German)
Features
- High sensitivity of detection - 0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe.
- Low background due to optimized washing and blocking procedures.
- Non-radioactive detection eliminates costs of radioisotopes and their disposal.
- Fast - ready-to-use components.
Description
The Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications such as Southern, Northern, dot and slot blotting, as well as screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods including the Biotin DecaLabel™ DNA Labeling Kit. Biotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes then are visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Therefore, visualization does not require X-ray film or other specific equipment.
The Blocking reagent and Blocking/Washing buffers are specifically designed to minimize background, which is often associated with biotin detection in conventional systems.
The positively charged SensiBlot™ Plus Nylon Membrane is highly recommended as a component of this detection system to ensure the highest sensitivity of detection and the lowest background.
Chromogenic detection of biotin-labeled probe.Applications
Southern, Northern, dot/slot blots, plaque or colony screening.Quality Control
The kit is tested in dot-blot hybridization with biotin-labeled probe.Components of the Kit
- Streptavidin-AP Conjugate
- BCIP/NBT Solution
- Blocking Reagent
- 10X Blocking/Washing Buffer
- 10X Detection Buffer
- Detailed Protocol (in pdf, ~40 KB):
Detection of biotin-labeled probe in dot-blot hybridization prepared with the Biotin DecaLabel™ DNA Labeling Kit.
Biotin-labeled lambda DNA/HindIII, prepared with Biotin DecaLabel™ DNA Labeling Kit, was used as a hybridization probe in the dot-blot of homologous DNA on the SensiBlot™ Plus Nylon Membrane.
Related Products
| Home | Search | Contacts | Order | Catalog | Support |
Updated kovo 04, 2008 11:17
