ZipRuler™ Express DNA Ladders, ready-to-use, 100-20000 bp
For fast sizing of wide range (100-20,000 bp) of DNA fragmentsFeatures
- Fast sizing of a wide range (100-20,000 bp) of DNA fragments.
- Robust when used in two different lanes of the same gel and run under fast electrophoresis conditions. The band pattern is not affected by the composition of the electrophoresis buffer, voltage or gel percentage.
- Fast separation (10-15 min) under various electrophoresis conditions (see table below).
- Precise when used in one lane and run under the conventional electrophoresis conditions.
- Allows approximate DNA quantification.
- Ready-to-use – premixed with a DNA loading dye for direct loading.
- Produce sharp bands.
- Easy-to-remember fragment sizes, bright reference bands.
- Stable at room temperature for 6 months.
Description & Other Information
Range Selection Guide
Product List:
- ZipRuler™ Express DNA Ladder Set, ready-to-use (100-20,000 bp)
- ZipRuler™ Express DNA Ladder 1
- ZipRuler™ Express DNA Ladder 1
ZipRuler™ Express DNA Ladder Set, ready-to-use
Designed for fast and accurate broad range (100-20,000 bp) DNA sizing and quantification on agarose gels under various electrophoresis conditions (see Table).
ZipRuler™ Express DNA Ladder Set, ready-to-use, is supplied with 6X Orange DNA Loading Dye.
ZipRuler™ Express DNA Ladder Set Ladder
formatCatalog
#Certificate
of Analysis
(in pdf)Product
resources
(in pdf)Concentration,
µg/µlAmount,
µgApplications Loading,
µg(µl)/laneRange,
bpFragments Agarose,
%ZipRuler™ Express DNA Ladder 1 Ready-to-use>> #SM1373 0.1 50 100-200 0.25-0.5(2.5-5) 100-10000 9 0.7-2.0 ZipRuler™ Express DNA Ladder 2 200-20000
Range
ZipRuler™ Express DNA Ladder 1 (9 fragments in bp): 10000, 5000, 3000, 2000, 1200, 850, 500, 300, 100.
ZipRuler™ Express DNA Ladder 2 (9 fragments in bp): 20000, 7000, 4000, 2500, 1500, 1000, 700, 400, 200.Table. ZipRuler™ Express DNA Ladder Separation Guide for Various Electrophoresis Conditions.
Description
ZipRuler™ Express DNA Ladder Set contains two ladders: ZipRuler™ Express DNA Ladder 1 and ZipRuler™ Express DNA Ladder 2 designed for fast and accurate broad range (100-20,000 bp) DNA sizing and quantification on agarose gels under various electrophoresis conditions. The ladders are mixtures of chromatography-purified individual DNA fragments.
The ZipRuler™ Express DNA Ladders 1 and 2 are recommended for loading into two different slots of the same gel to evaluate a wide range of DNA bands during short electrophoresis runs. Electrophoresis can also be performed within a wide voltage range, and the agarose concentration may vary within 0.7-2% (see table above).
Another approach is to load equal amounts of both ladders into the same gel slot. Three reference bands will then be formed during electrophoresis of the complexed ZipRuler™ Ladder. However, it takes more time for the accurate separation of the complexed ZipRuler™ bands.
ZipRuler™ Express DNA Ladder 1, ready-to-use, is premixed with the 6X Orange DNA Loading Dye, whereas ZipRuler™ Express DNA Ladder 2, ready-to-use, is premixed with the 6X MassRuler™ DNA Loading Dye. When both ladders are loaded in the same gel slot, the migration of DNA is monitored by three electrophoresis tracking dyes (bromophenol blue, xylene cyanol FF and orange G), whereas when applied alone ZipRuler™ Express DNA Ladder 1 has two tracking dyes (orange G and xylene cyanol), and ZipRuler™ Express DNA Ladder 2 has bromophenol blue.
The set is supplied with 6X Orange DNA Loading Dye for sample DNA.Storage and Loading Buffer (for ZipRuler™ Express DNA Ladder 1)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.Storage and Loading Buffer (for ZipRuler™ Express DNA Ladder 2)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.6X Orange DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Load the ZipRuler™ Express DNA Ladder 1 and the ZipRuler™ Express DNA Ladder 2 in two different lanes of the gel.
- Dilute your DNA sample with the appropriate DNA loading dye (supplied with the ladder): mix 1 volume of the dye solution with 5 volumes of the DNA solution.
- Do not heat before loading.
Quality Control
Tested in appropriate gel electrophoresis. The DNA concentration determined spectrophotometrically. The absence of nucleases is confirmed by appropriate tests.
Storage
Store at room temperature or at +4°C for 6 months. For longer periods, store at -20°C.
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Updated vasario 01, 2008 09:31
