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TopVision™ LM GQ Agarose (low melting point)

#R0801 25g

Related Documents (in pdf, ~150 KB):

Certificate of Analysis: #R0801
MSDS (English)
MSDS (English-USA)
MSDS (German)

1% gel, 1X TAE buffer
1 - GeneRuler™ DNA Ladder Mix, 1 µg/lane
2 - GeneRuler™ 100 bp Plus DNA Ladder, 1 µg/lane
3 - MassRuler™ DNA Ladder Mix, ready-to-use, 20 µl/lane
4 - GeneRuler™ 1 kb DNA Ladder, 1 µg/lane
5 - MassRuler™ High Range DNA Ladder, ready-to-use, 20 µl/lane
6 - GeneRuler™ 100 bp DNA Ladder, 1 µg/lane

Description
TopVision™ LM GQ Agarose is a highly purified low melting temperature agarose suitable for analytical and preparative electrophoresis of nucleic acids.

Features
  • GQ (Genetic Quality) certified, which ensures that in-gel applications can be performed in remelted agarose, avoiding DNA extraction steps.
  • Ideal for digestion by agarase, which ensures efficient recovery of large DNA fragments suitable for cloning or enzymatic processing.
  • Low DNA binding.
  • DNA recovered from gels can be used in enzymatic processing (restriction, ligation, etc.).
Properties
  • Electroendosmosis EEO (1) 
  • Gel strength (1% gel)  
  • Gelling temperature 
  • Melting temperature 
  • Moisture 
 <0.12
>250g/cm2
24-28°C
<65.5°C
<7%
Applications
  • Analytical electrophoresis of nucleic acids.
  • Preparative electrophoresis.
  • In-gel enzymatic processing (digestion, ligation etc.).

Quality Control
Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases.

Note

Can be used at concentrations between 0.7-2% with all typical buffer systems.

Related Products
Agarase #EO0461
DNA Extraction Kit #K0513
50X TAE Buffer #49

Reference

  1. Wieme, R.J., Agar gel electrophoresis. Elsevier, Amsterdam, 1965.

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Updated vasario 01, 2008 10:31